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Improving Mineral Performance of
by Using a New Mineral Delivery System
The creation of a new complexing environment for minerals that combines the benefits of amino acid chelation and oligofructose complexing (AAOF).
© 2009 Melaleuca, Inc.
Improving Mineral Performance of
by Using a New Mineral Delivery System
›› Results and Discussion
…oligofructose may significantly improve mineral absorption.
Note: The results of this work have been filed as a patent application. They were presented at the 15th annual meet ing of the Society for Free Radical Biology and Medicine (Abstract, in the journal Free Radical Biology and Medicine13).
The complete findings are currently being prepared for publication in a journal article. This document provides a brief overview of those findings.
Alexander B. Rabovsky—Research & Technology
Development, Melaleuca, Inc.
A.M. Komarov—Department of Biochemistry,
George Washington University
Jeremy Ivie—Research & Technology Development,
Garry R. Buettner—Free Radical and Radiation
Biology, University of Iowa
Is it possible to increase the solubility of the minerals typically included in dietary supplements while, at the same time, decreasing the rate of free radical generation triggered by these minerals?
Minerals are essential for good health. Every living cell depends on minerals for proper structure and function. Minerals are needed for the formation of tissue and bones, healthy nerve function, and proper operation of the cardiovascular system.
As a result, a daily multivitamin/multimineral supplement is recommended to help ensure proper nutrient levels.
But unlike minerals in natural foods that are incorporated in bioorganic structures, minerals in dietary supplements are usually in an inorganic form: sulfates, chlorides, oxides, etc. Unfortunately, the majority of minerals in these forms precipitate
(fall out of solution and solidify) at the neutral pH of the small intestine, making absorption questionable.
In addition, some minerals act as a free radical catalyst, triggering the generation of massive amounts of free radicals that can neutralize the effectiveness of the antioxidants (like vitamins C and E) found in the supplement. The minerals primarily responsible for accelerating the generation of free radicals are copper (sometimes
listed as cupric on ingredient labels) and iron (often listed as ferrous on ingredient labels).
Results and Discussion
Understanding the problems inherent in mineral supplementation, many have attempted to replicate the mineral form naturally found in fruits, vegetables, and other whole foods. These attempts include complexing them with salts from organic acids (citrates, gluconates, and fumarates) as well as amino acid chelation and Melaleuca’s proprietary Fructose Compounding.
While looking for ways to improve upon this, we found research documenting that some forms of fiber—namely oligofructose—may significantly improve mineral absorption in animals and
Building upon those findings, a new complexing environment for minerals has been created that combines the benefits of amino acid chelation and oligofructose complexing (AAOF).
The stomach is very acidic, with a pH close to 1. In this low pH environment, the majority of minerals are soluble. The problem is that absorption takes place in the small intestine, which has a neutral pH, from 7.0–7.2. In this state, many minerals precipitate or fall out of solution, making them
2 A New Mineral Delivery System
Solubility is at its lowest in inorganic forms of minerals.
Organic forms proved better, but the new AAOF form brought the highest levels of solubility.
›› Free Radical Generation
The ability of minerals to trigger free radicals differs greatly depending on the mineral form.
difficult to absorb.
Using the AAOF complex, the solubilities of key minerals, including calcium, magnesium, copper, iron, manganese, chromium, molybdenum, and selenium were measured. Each substance was first dissolved in acidic stomach conditions. The acidity was then adjusted to mirror pH conditions in the intestine. The amount of mineral still remaining in solution was then measured. All tested minerals were almost 100% soluble.
To measure the solubility of the final supplement form, the AAOF complex was compared to seven popular commercial products, using the same method.
Testing entire supplement formulas that minerals were put into created complications and interference that resulted in drastically different levels of solubility. So the exact same mineral form was found to be more soluble in one supplement formulation, and much less soluble in another formulation (see Figure 1).
One A Day® (Sulfate)
One A Day® (Sulfate)
Figure 1. The solubility of copper and zinc at the conditions of the small intestine (pH 7.0–7.2) after exposure of tablets at the conditions of the stomach (pH 1.0).
Solubility was found to be at its lowest in inorganic forms of minerals. Organic forms proved better, but the new AAOF form brought the highest levels of solubility.
Free Radical Generation
Free radicals are highly reactive molecules with lifespans as short as one-billionth of one second. An overabundance of free radicals is considered dangerous because they have the ability to damage the molecules and tissues of the body. In fact, the majority of degenerative diseases can be linked to free radical damage.
Minerals (especially copper and iron) are known triggers of free radical generation, but their ability to trigger free radicals differs greatly depending on the mineral form.
The main technology for studying free radicals is electron paramagnetic resonance (EPR), also known as electron spin resonance (ESR). Many
experimental models use this technology. This study utilized a few key models:
•Ascorbateradicalmodel(oxidationof vitamin C)5
•Hydroxylradicalmodel(Fentonreaction,spin trapping technique)6, 11
•Tocopherylradicalmodel(oxidationof vitamin E)8, 16
For demonstration purposes, the results from the ascorbate radical model will be outlined.
Ascorbate Radical Model
The oxidation of ascorbic acid catalyzed by iron or copper first leads to the formation of ascorbate radical.
Below is the EPR signal of the ascorbate radical. The amplitude of the waveform goes down as the ascorbic acid (vitamin C) is consumed.
Figure 2. EPR signal of ascorbate radical.
3 A New Mineral Delivery System
›› Ascorbate Radical Model
Ascorbic acid (vitamin C) survives much longer when complexed with AAOF.
›› DCF Loss Model
Copper chelate behaved much better than copper (sulfate) and copper gluconate. But AAOF complex demonstrated the lowest oxidative ability.
Figure 3 shows the loss of ascorbate radicals over time in the presence of different mineral forms.
|•Iron Sulfate •Iron AAOF|
|•Copper Sulfate •Copper AAOF|
Figure 3. The rate of loss of the ascorbate radical is much faster when in the presence of the inorganic forms of iron or copper.
Free Radical Oxidation of Indicator Dyes
In this process, different forms of copper were mixed with hydrogen peroxide and indicator dye(dichlorodihydrofluoresceinorDCF).DCF, after being oxidized by free radicals, turns to
an orange pigment, which can be measured spectrophotometrically. The intensity of this orange color was measured by its absorbance at 500 nm (OD-500 nm). The results can be seen in Figure 4.
Free Radical Oxidation of Dye
Figure 4. The sulfate form of copper brought about the most rapid oxidization of DCF; the oligofructose complex form had the least oxidation.
This illustrates that ascorbic acid (vitamin C) survives much longer when complexed with AAOF. In about 30 minutes, the traditional forms of copper and iron destroy almost all of the ascorbic acid, but the AAOF form remains stable at about 60%.
We used the following methods to measure the oxidative damage caused by free radicals:
•Lossoftrolox(awater-solubleanalogof vitamin E) in solution16
Typical experiments showing the loss of vitamin C are shown in figure 3. In figure 4 we show typical experimental results of the free radical-mediated oxidation of indicator dyes.
The inorganic form of copper (sulfate) has the highest oxidative ability. Surprisingly, copper gluconate (which is an organic form of copper) generated almost the same amount of free radicals as sulfate. Copper chelate behaved much better, and AAOF complex demonstrated the lowest oxidative ability.
When a substance oxidizes it will consume the oxygen dissolved in the water of a solution. To analyze the rate of oxidation of the entire multivitamin/multimineral supplement, an
oxygen-monitoring model was used. Because this model measures the loss of dissolved oxygen,
it measures all oxidative loss regardless of the individual ingredient source.
A commercially available men’s multivitamin/ multimineral supplement using copper oxide was compared with Melaleuca’s multivitamin/ multimineral supplement containing copper AAOF complex.
4 A New Mineral Delivery System
›› Oxygen- Monitoring Model
This model measures all oxidative loss, regardless of the individual ingredient source.
|Copper Oxide||Copper AAOF|
Figure 5. Rate of oxygen consumption for the two supplements.
A similar concentration of copper was taken from each formula and combined with identical amounts of ascorbic acid (vitamin C). These formulas were then dissolved in acidic stomach conditions (pH 1). The acidity was then adjusted to mirror conditions in the intestines (pH 7.0–7.2). The oxygen concentration in the solution was then measured electrochemically with a Clark electrode.
The oxygen consumption rate was more than four times greater for the copper oxide supplement (315 pmol/sec) than it was for the AAOF complex supplement (75 pmol/sec).
•Comparedtotraditionalforms(sulfates,chlorides, oxides, etc.) of minerals (copper, iron, and
zinc), Melaleuca’s AAOF mineral form delivers significantly higher levels of solubility.
•Melaleuca’sAAOFmineralformsignificantly reduces the rate of free radical generation compared to traditional mineral forms.
•Melaleuca’sAAOFmineralformdrasticallylowers oxidative loss of vitamins and other key nutrients
compared to traditional mineral forms.
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